Fungal infection in mice

Fungal LD90 tests are conducted to characterise the infectivity of a fungus (e.g. Aspergillus) or to evaluate the effectiveness of antifungal treatments. For example, the antifungal treatment may be started 24 hours after infection and continued for seven days, with mortality then recorded over the following week.

In a study to evaluate the potential to use body temperature as a predictor of death in fungal (Candida) LD90 tests, male CD1 mice (4 to 5 weeks old and between 18 and 20 g) were immunosuppressed with cyclophosphamide and infected with either C. albicans, C. guillermondii or C. glabrata. Body temperature was measured using an implantable transponder and a high performance non-contact thermometer.  The non-contact thermometer gave reliable results and enabled an endpoint of 33.3 oC to be defined, which would have been able to predict mortality in 67 per cent of the mice.

Source: Warn et al. (2003) Laboratory Animals 37: 126-131

This builds on earlier work on humane endpoints in rodent infection studies see: Acred et al. (1994) Laboratory Animals 28, 13-18